Contaminant Guide

Total Coliform in Well Water

Total Coliform in private well water: where it comes from, what the limits are, how to test for it, and which treatment methods actually remove it.

Where total coliform comes from

  • surface water infiltration through shallow or poorly cased wells
  • nearby septic systems or leach fields
  • agricultural runoff and manure application
  • wildlife fecal contamination of wellhead area
  • failed or cracked well casing or grouting
  • flooding events contaminating wellhead

Health considerations

  • indicator of fecal contamination pathway — Total coliform bacteria are not themselves harmful at typical concentrations, but their presence in a well signals a pathway for fecal pathogens. EPA MCL: no more than 5% of monthly samples may be total coliform positive (public systems). Private well standard: should be absent (0 CFU/100 mL).
  • gastrointestinal illness (if fecal pathogens present) — Total coliform-positive wells carry elevated risk of harboring Giardia, Cryptosporidium, norovirus, or pathogenic E. coli. A positive total coliform result should trigger follow-up E. coli testing and well inspection.

EPA MCLG = 0 for total coliform (public water systems). For private wells, no regulatory standard exists, but the public health guidance is absence of total coliform. A positive result should be followed immediately by E. coli testing — the presence of total coliform without E. coli still warrants investigation and likely well disinfection.

Who is most at risk

  • infants and young children
  • elderly adults
  • immunocompromised individuals
  • pregnant women
  • households within 100 feet of a septic system or livestock operation

Testing

EPA Method 9222B (membrane filtration), IDEXX Colilert presence/absence or MPN method; results reported as CFU/100 mL or present/absent; certified lab required; sample must be collected in sterile container with sodium thiosulfate preservative

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Treatment

  • UV ultraviolet disinfection (whole-house, NSF/ANSI 55 certified)
  • continuous chlorination (chemical feed pump + contact tank)
  • shock chlorination (one-time well treatment for episodic contamination)
  • well casing inspection and repair (address root cause)
  • reverse osmosis (point-of-use for drinking water only)

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Regulatory framework

No federal MCL · MCLG: 0 µg/L

EPA MCLG = 0 for total coliform (public water systems). For private wells, no regulatory standard exists, but the public health guidance is absence of total coliform. A positive result should be followed immediately by E. coli testing — the presence of total coliform without E. coli still warrants investigation and likely well disinfection.

Detection

EPA Method 9222B (membrane filtration), IDEXX Colilert presence/absence or MPN method; results reported as CFU/100 mL or present/absent; certified lab required; sample must be collected in sterile container with sodium thiosulfate preservative

Occurrence

Most commonly reported in: Michigan, Pennsylvania, Ohio, Wisconsin, New York, North Carolina, Virginia, Tennessee.

  • shallow unconfined aquifer
  • fractured bedrock
  • karst limestone
  • alluvial

Data access

Access our data API and methodology

References

  1. Borchardt, M.A., Bertz, P.D., Spencer, S.K., & Battigelli, D.A. (2003). Incidence of enteric viruses in groundwater from household wells in Wisconsin. Applied and Environmental Microbiology, 69(2), 1172-1180. https://doi.org/10.1128/AEM.69.2.1172-1180.2003
  2. Wallender, E.K., Ailes, E.C., Yoder, J.S., Roberts, V.A., & Brunkard, J.M. (2014). Contributing factors to disease outbreaks associated with untreated groundwater. Groundwater, 52(6), 886-897. https://doi.org/10.1111/gwat.12121